![]() ![]() Hematopoietic cells is associated with increased cell proliferationĪnd/or survival, the Epo/EpoR signaling pathway in tumor cells does Tumor cells, with or without the stimulatory effect of Epo on theseĬells ( 10– 16). The presence of EpoR has been confirmed in numerous Transfusion in cancer-related anemia ( 7), a frequent symptom of cancer ( 8) due to chemotherapy ( 9). Treatment of anemia resulted from chronic liver diseases ( 5) or chronic renal failure ( 6), and is a good alternative to blood Recombinant human Epo (rhEpo) is currently available for the The Epo hormone facilitates the creationĪnd maturation of erythroid precursors, and stimulatesĮrythropoiesis upon binding to its receptor, known as Glycoprotein produced in the kidney by neural peritubularįibroblasts and by the outer medulla ( 1, 2), depending Furthermore, modified immunoprecipitation of EpoR followed by matrix‑assisted laser desorption/ionization‑time‑of‑flight mass spectrometry analysis confirmed a 57 kDa protein as a human Epo-interacting protein in both cell lines.Įrythropoietin (Epo) is a low-molecular weight Applying this technique, the presence of EpoR and its interaction with Epo in human ovarian adenocarcinoma A2780 and normal human umbilical vein endothelial cells was confirmed. With the aim of bypassing the direct detection of EpoR with an anti-EpoR antibody, the present authors propose a far‑western blot methodology, which in addition, confirms the interaction of Epo with EpoR. This apparent discrepancy is based, according to certain authors, on the use of non‑specific anti‑EpoR antibodies. By contrast, a previous study claims the absence of EpoR in tumor cells. Numerous studies have reported pro‑stimulating and anti‑apoptotic effects of Epo in cancer cells, thus demonstrating EpoR functionality in these cells. The fact that EpoR was also detected in neoplastic cells has opened the question about the relevance of anemia treatment with recombinant Epo in cancer patients. The interaction between erythropoietin (Epo) and EpoR is important for the production and maturation of erythroid cells, resulting in the stimulation of hematopoiesis. The erythropoietin receptor (EpoR) is a member of the cytokine receptor family.
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